{"id":20,"date":"2019-04-10T04:47:19","date_gmt":"2019-04-10T04:47:19","guid":{"rendered":"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/?post_type=chapter&#038;p=20"},"modified":"2019-04-10T05:05:10","modified_gmt":"2019-04-10T05:05:10","slug":"prelude-carbohydrates-part-a-mono-oligosaccharides","status":"publish","type":"chapter","link":"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/chapter\/prelude-carbohydrates-part-a-mono-oligosaccharides\/","title":{"rendered":"1A. Prelude: Carbohydrates (Part-A) \u2013Mono &amp; Oligosaccharides"},"content":{"raw":"<div><span style=\"float: right\"><a href=\"https:\/\/youtu.be\/mW1nQkhQSIA\" target=\"_blank\" rel=\"noopener\"><img src=\"http:\/\/epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/2018\/11\/download.png\" alt=\"epgp books\" width=\"75px\" height=\"75px;\" \/><\/a>\r\n<\/span><\/div>\r\n<strong>\u00a0 \u00a0<\/strong>\r\n\r\n&nbsp;\r\n\r\n&nbsp;\r\n\r\n&nbsp;\r\n\r\n<strong> PRELUDE-CARBOHYDRATES-<\/strong>\r\n\r\n&nbsp;\r\n\r\n<strong>(Monosaccharide\u2019s and Oligosaccharides)<\/strong>\r\n\r\n&nbsp;\r\n\r\n<strong>Objectives<\/strong>\r\n<ol>\r\n \t<li>Introduction and the function of Functions of carbohydrates<\/li>\r\n \t<li>To understand classification of carbohydrates<\/li>\r\n \t<li>To study the characteristics of carbohydrates<\/li>\r\n \t<li>To understand the properties of mono- and di- saccharides:<\/li>\r\n<\/ol>\r\n<strong>\u00a0 \u00a0<\/strong>\r\n\r\n<strong>Introduction<\/strong>\r\n<ul>\r\n \t<li style=\"text-align: justify\">Carbohydrates are one of the three major macronutrients in our diet representing a wide group of substances which include the sugars, starches, glycogen, gums and celluloses. Carbohydrates are known as the important energy source for living things.<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li style=\"text-align: justify\">They comes from the process of photosynthesis, an endothermic reaction in which condensation of carbon dioxide takes place and which requires light energy and the pigment chlorophyll.<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li style=\"text-align: justify\">The chemical component of carbohydrates is carbon, hydrogen, and oxygen.<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li style=\"text-align: justify\">Generally, but not always the hydrogen and oxygen in carbohydrates are present in the proportion oftwo hydrogen atoms to one oxygen atom as in H2O, from which fact the term \u201ccarbohydrates\u201d was derived<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li style=\"text-align: justify\">The carbohydrates are actually or potentially hydroxy aldehydes or ketones, and in most cases they are polyhydroxy aldehyde or ketones compounds that can be hydrolyzed to them. It also means \u201chydrate of carbon\u201d<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li style=\"text-align: justify\">Plant biomass is made up of three fourths of carbohydrates but in animal body it is present in small quantities. In animal it is stored in the form of glycogen, sugar and their derivatives<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li>All living organism contains derived monosaccharides such as the sugar acids, amino sugars and the deoxysugars<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li>Derivatives of the carbohydrates also contain nitrogen, phosphates and sulfur compounds. Carbohydrates can combine with lipid and protein to form glycolipids and glycoproteins.<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li style=\"text-align: justify\">Carbohydrates and their derivatives are present in all animal tissues and tissue fluids, blood and milk. The glucosides are a class of carbohydrates are derivatives which are frequent constituents of plants<\/li>\r\n<\/ul>\r\n<ul>\r\n \t<li style=\"text-align: justify\">The major components of living matter is formed from carbohydrates, proteins and fats<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li style=\"text-align: justify\">This complex form of carbohydrates is required for the maintenance of cell structure and functions<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li>Presence of functional group like alcohol on almost every carbon makes the chemistry of carbohydrates a complex structure<\/li>\r\n<\/ul>\r\n<ul>\r\n \t<li style=\"text-align: justify\">Carbohydrate may be exist in either a straight chain or a closed ring structure. The general reaction between the aldehyde and ketone part of the carbohydrates and alcohol groups forms the derivative called hemiacetals and hemiketals leads to the formation of ring structure. The most common basic structure of carbohydrates is closed rings Glucose is an example of a single closed ring carbohydrate<\/li>\r\n<\/ul>\r\n&nbsp;\r\n\r\n<strong>Functions of carbohydrates<\/strong>\r\n<ul>\r\n \t<li style=\"text-align: justify\">The simplest carbohydrates are initially synthesized in plants by the process of photosynthesis which is a series of reactions.<\/li>\r\n<\/ul>\r\n&nbsp;\r\n\r\n<strong>Carbohydrate functions as\u2026<\/strong>\r\n<ul>\r\n \t<li>bio Fuel<\/li>\r\n \t<li>primary Source of Energy<\/li>\r\n \t<li>storage food<\/li>\r\n \t<li>framework in body<\/li>\r\n \t<li>anticoagulant<\/li>\r\n \t<li>antigen<\/li>\r\n \t<li>hormone<\/li>\r\n \t<li>provide raw material for industry<\/li>\r\n \t<li>Essential for Fat Oxidation<\/li>\r\n \t<li>plays role in gastro-intestinal function<\/li>\r\n \t<li>add flavour to the diet<\/li>\r\n \t<li>play an important role in molecular recognition<\/li>\r\n<\/ul>\r\n&nbsp;\r\n\r\n&nbsp;\r\n\r\n<strong>Classification<\/strong>\r\n<ul>\r\n \t<li style=\"text-align: justify\">The carbohydrates are sometimes referred to as the saccharides<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li>The word saccharides comes from the Greek word sakcharon meaning sugar<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li style=\"text-align: justify\">Those carbohydrates such as glucose and fructose that cannot be hydrolyzed into simpler compounds are called simple sugars or <em>monosaccharides<\/em><\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li style=\"text-align: justify\">Certain carbohydrates are made of two molecules of monosaccharides such as sucrose, lactose and maltose<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li>These carbohydrates are called <em>dissarcharides.<\/em> Similarly, trisaccharides are composed of three and tetrasaccharides of four monosaccharides molecules<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li style=\"text-align: justify\">All these compound carbohydrates may be readily hydrolyzed into their constituent monosaccharides by heating with dilute acids or by the action of specific enzymes<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li style=\"text-align: justify\">According to the number of C atoms and functional groups in its simplest form of carbohydrates are further classified<\/li>\r\n<\/ul>\r\n<ul>\r\n \t<li style=\"text-align: justify\">The suffix \u2013ose indicates the types of sugar.<\/li>\r\n<\/ul>\r\n&nbsp;\r\n\r\n<strong><em>Nomenclature of carbohydrates based on number of carbon atoms<\/em><\/strong>\r\n<ol>\r\n \t<li>Triose= 3 carbon atom(Glyceraldehyde, Dihydroxyacetone<\/li>\r\n \t<li>Tetrose= 4 carbon atom(Erythrose)<\/li>\r\n \t<li>Pentose= 5 carbon atom(Ribose, Ribulose, Xylulose)<\/li>\r\n \t<li>Hexose=6 carbon atom(Glucose, Galactose, Mannose, Fructose)<\/li>\r\n \t<li>Heptose=7 carbon atom(Sedoheptulose) and so on<\/li>\r\n<\/ol>\r\n<img class=\"aligncenter size-full wp-image-21\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1.png\" alt=\"\" width=\"559\" height=\"309\" \/>\r\n\r\n&nbsp;\r\n\r\n<strong>Characteristics of carbohydrates<\/strong>\r\n\r\n&nbsp;\r\n\r\n<strong><em>Isomerism<\/em><\/strong>\r\n\r\n&nbsp;\r\n<p style=\"text-align: justify\">Isomers are defined as molecule with the same chemical formula and often with the same kinds of chemical bonds between atoms, but in which the atoms are arranged differently i.e. they have different structural formulae. Isomerism can be of two types: Structural isomerism and Stereoisomerism<\/p>\r\n&nbsp;\r\n\r\n<img class=\"aligncenter size-full wp-image-39\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-15.png\" alt=\"\" width=\"500\" height=\"298\" \/>\r\n\r\n&nbsp;\r\n\r\n<strong>Monosaccharides <\/strong>Greek, <em>mono<\/em>=one; <em>sakchron<\/em>=sugar.\r\n<ul>\r\n \t<li style=\"text-align: justify\">Monosaccharides are the simplest sugar formed by the single molecules which furthercannot be hydrolyzed into smaller units. Depending upon the number of carbon arrangement in a unit, mono saccharides are subdivided into aldoses and ketoses.<\/li>\r\n<\/ul>\r\n<ul>\r\n \t<li>The aldoses and ketoses are represented by the following general formulas.<\/li>\r\n<\/ul>\r\n<img class=\"aligncenter size-full wp-image-23\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-2.png\" alt=\"\" width=\"349\" height=\"237\" \/>\r\n<ul>\r\n \t<li style=\"text-align: justify\">Study of the chemical and physical properties of many sugar has shown the cyclic forms predominate over open chain structure, both in solution and in the solid state.<\/li>\r\n<\/ul>\r\n<ul>\r\n \t<li style=\"text-align: justify\">Alcohol may react with aldehyde and ketone moieties of the carbohydrates to form an unstable compounds called hemiacetals or hemiketals, respectively. This is a reaction between two molecules, i.e., it is intermolecular<\/li>\r\n<\/ul>\r\n<img class=\"aligncenter size-full wp-image-24\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-3.png\" alt=\"\" width=\"379\" height=\"214\" \/>\r\n<ul>\r\n \t<li style=\"text-align: justify\">In glucose the \u2013OH on carbon 5 (C-5) can react intramolecularly with carbonyl group (on C-1) to form a stable, cyclic hemiacetal. The cyclic form of glucose is a six-membered ring; such sugars are called <strong>pyranose<\/strong> because they resemble pyran. Fructose forms a five membered ring called <strong>furanose<\/strong> as it resembles furan.<\/li>\r\n<\/ul>\r\n<img class=\"aligncenter size-full wp-image-25\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-4.png\" alt=\"\" width=\"324\" height=\"131\" \/>\r\n<ul>\r\n \t<li style=\"text-align: justify\">The pyranose and furanose ring structure of sugar proposed by Haworth more accurately represent their actual configuration than do the older projection structures used by Fischer and other workers, though the older ring forms are still used extensively in writing reaction of sugars.<\/li>\r\n<\/ul>\r\n<img class=\"aligncenter size-full wp-image-26\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-5.png\" alt=\"\" width=\"368\" height=\"212\" \/>\r\n<ul>\r\n \t<li style=\"text-align: justify\">The pyranose ring of a six carbon aldose sugar can exist in either a boat or a chair configuration<\/li>\r\n<\/ul>\r\n<ul>\r\n \t<li style=\"text-align: justify\">The substituents attached to the ring carbon thatextend parallel to the symmetry are said to be axial (a) whereas those that extend outward from the axis are said to be equatorial (e).<\/li>\r\n<\/ul>\r\n<img class=\"aligncenter size-full wp-image-27\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-6.png\" alt=\"\" width=\"420\" height=\"177\" \/>\r\n\r\n<strong><em>Stereoisomerism<\/em><\/strong>\r\n<ul>\r\n \t<li style=\"text-align: justify\">Many of the carbohydrates contain the same number of atoms and the same kinds of groups, yet definitely distinct substances<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li style=\"text-align: justify\">For example, the formula C6H12O6 represents 16 differently simple sugars all processing the structure CH2OH.CHOH.CHOH.CHOH.CHOH.CHO<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li style=\"text-align: justify\">This is due to differently arrangement of the constituent groups of the molecules in space. This phenomenon represents what is called stereoisomerism (space isomerism) and these sugars are stereoisomers.<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li>An asymmetric carbon atom is a carbon when it is attached to four different types of atoms or groups<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li style=\"text-align: justify\">When there are several asymmetric carbon atoms in a chain molecule and the end group<span style=\"text-align: initial;font-size: 1em\"> are not identical, the number of stereoisomers possible is equal to 2n, where n is the number of asymmetric carbon atoms<\/span><\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li style=\"text-align: justify\">For example glucose contains 4 asymmetric carbon and thus are 24= 16 isomers. The simplest monosaccharides is glyceraldehydes<\/li>\r\n<\/ul>\r\n<ul>\r\n \t<li style=\"text-align: justify\">Glyceraldehyde has a single asymmetric carbon atom (the central one) and so two <strong>stereoisomers <\/strong>(also called<strong> optical isomers<\/strong>) are possible, that is two forms of glyceraldehyde, denoted as <strong>D<\/strong>- and <strong>L-glyceraldehyde<\/strong>, which are mirror images of each other<\/li>\r\n<\/ul>\r\n<img class=\"aligncenter size-full wp-image-28\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-7.png\" alt=\"\" width=\"320\" height=\"191\" \/>\r\n\r\n&nbsp;\r\n<ul>\r\n \t<li style=\"text-align: justify\">It has been possible to relate the spatial configuration of sugars, amino acids and many other optically active substances of biological importance to the configuration of the dextro and levo forms of glyceraldehydes<\/li>\r\n<\/ul>\r\n<ul>\r\n \t<li style=\"text-align: justify\">Monosaccharides can be named by a system that is based on the number of carbons with the suffix \u2013ose added. There is also a system for numbering the carbons. They are numbered sequentially, with the aldehyde or ketone group being on the carbon with the lowest possible number<\/li>\r\n<\/ul>\r\n&nbsp;\r\n\r\n<strong><em>Optical isomerism<\/em><\/strong>\r\n<ul>\r\n \t<li style=\"text-align: justify\">When a beam of plane polarized lightthrough the solution of a non-racemic mixture, rotation of polarization occurs<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li>Carbohydrates contain asymmetric carbons, because of this carbohydrates are optically active, and rotating the plane polarized light<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li style=\"text-align: justify\">The compounds which rotates the plane polarized light to the right (clockwise) is called dextrorotatory (indicated by d or +)and the compound which rotates the plane polarized light to the left (counter clockwise) is called levorotatory (indicated by l or -).<\/li>\r\n<\/ul>\r\n<p style=\"text-align: justify\"><img class=\"aligncenter size-full wp-image-29\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-8.png\" alt=\"\" width=\"587\" height=\"230\" \/><\/p>\r\n<strong><em>Enantiomers<\/em><\/strong>\r\n<ul>\r\n \t<li style=\"text-align: justify\">An Enantiomers is one of two stereoisomers that are mirror images of each other and are non- superimposable<\/li>\r\n<\/ul>\r\n<img class=\"aligncenter size-full wp-image-30\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-9.png\" alt=\"\" width=\"526\" height=\"210\" \/>\r\n\r\n&nbsp;\r\n\r\n<strong><em>Epimers<\/em><\/strong>\r\n<ul>\r\n \t<li style=\"text-align: justify\">Epimers are those sugars which differ with its isomers in configuration at only one asymmetric carbon<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li>D-glucose and D-galactose contains four asymmetric groups, and that all these groups are identical except the configuration at C-4<\/li>\r\n<\/ul>\r\n<ul>\r\n \t<li style=\"text-align: justify\">Such isomers are called are epimersTheprocess of interconversion of epimers is known as epimerization and <em>epimerases<\/em> catalyzes this reaction<\/li>\r\n<\/ul>\r\n<img class=\"aligncenter size-full wp-image-31\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-10.png\" alt=\"\" width=\"224\" height=\"238\" \/>\r\n\r\n&nbsp;\r\n\r\n<strong>Properties of Mono saccharides:<\/strong>\r\n<ul>\r\n \t<li>Mutarotation<\/li>\r\n \t<li>Glycoside Formation<\/li>\r\n \t<li>Reducing Power<\/li>\r\n \t<li>Reduction<\/li>\r\n \t<li>Oxidation with mild and strong oxidizing agent<\/li>\r\n \t<li>Methylation \/ Esterification<\/li>\r\n \t<li>Dehydration<\/li>\r\n \t<li>Osazoneformation with phenyl hydrazine.<\/li>\r\n<\/ul>\r\n&nbsp;\r\n\r\n<strong><em>Glucose<\/em><\/strong>\r\n<ul>\r\n \t<li>Glucose is an aldohexose \u2013<\/li>\r\n \t<li>It is a poly hydroxyaldehyde and contains 6-carbon<\/li>\r\n \t<li style=\"text-align: justify\">Carbohydrates are transformedinto simplest glucose which is the primary carbohydrates utilized by the body tissues.<\/li>\r\n \t<li>Glucose is the sugar of blood and other body fluids<\/li>\r\n \t<li style=\"text-align: justify\">Disaccharide like sucrose, maltose and lactose and polysaccharides such as starch, cellulose and glycogen contains glucose as one of the monomers<\/li>\r\n \t<li>Glucose is 25% less sweet than sucrose<\/li>\r\n \t<li>Blood normally contains from 60 to 90 mg of glucose in each 100ml<\/li>\r\n<\/ul>\r\n&nbsp;\r\n\r\n&nbsp;\r\n\r\n<strong><em>Galactose<\/em><\/strong>\r\n<ul>\r\n \t<li>Galactose is an aldohexose<\/li>\r\n \t<li>It is a poly hydroxyaldehyde and a 6-carbon containing compound<\/li>\r\n \t<li>It is found in dairy products, sugar beets, pectin and gum<\/li>\r\n \t<li>Galactose is one of the monomer of the disaccharide lactose<\/li>\r\n \t<li>Galactose is 80% less sweet than sucrose<\/li>\r\n \t<li>It is epimers of glucose<\/li>\r\n<\/ul>\r\n&nbsp;\r\n\r\n<strong><em>Fructose<\/em><\/strong>\r\n<ul>\r\n \t<li>Fructose is a ketohexose<\/li>\r\n \t<li>It is a poly hydroxyketone and contains 6-carbon<\/li>\r\n \t<li>It is found in fruit, flowers, root vegetables and honey<\/li>\r\n \t<li>Disaccharide sucrose contains fructose as one monomer<\/li>\r\n \t<li>It is the sweetest of all naturally occurring carbohydrates and 175% sweeter than sucrose<\/li>\r\n \t<li>Fructose has higher solubility than other sugars<\/li>\r\n<\/ul>\r\n&nbsp;\r\n\r\n<strong>Properties of oligosaccharides<\/strong>\r\n<ul>\r\n \t<li style=\"text-align: justify\">The oligosaccharides are composed of the disaccharides, trisaccharides and tetrasaccharides and are so designated to indicate the number of monosaccharides units involved in the structures<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li style=\"text-align: justify\">Disaccharides is a sugar formed when a bond between the first carbon of one sugar and a hydroxyl group at any position on the other sugar is formed and a molecule of water is removed<\/li>\r\n<\/ul>\r\n<ul>\r\n \t<li style=\"text-align: justify\">Sucrose, maltose, and lactose are the most important disaccharides. Each one of these on hydrolysis with either an acid or an enzyme gives two molecules of the monosaccharides by which it is made of.<\/li>\r\n<\/ul>\r\n&nbsp;\r\n\r\n<strong><em>Sucrose<\/em><\/strong>\r\n<ul>\r\n \t<li style=\"text-align: justify\">It is called as table sugar<\/li>\r\n \t<li style=\"text-align: justify\">Sucrose occurs especially in the juices of plants such as sugar beets, sugar cane sorgum, sugar maple and pine apple<\/li>\r\n \t<li style=\"text-align: justify\">Ripe fruits are rich in sucrose<\/li>\r\n \t<li style=\"text-align: justify\">20% of sugar cane is sucrose<\/li>\r\n \t<li style=\"text-align: justify\">It is non-reducing sugar, is relatively stable toward the action of alkali and in general does not give the reaction characteristic of the sugar groupand hence no reaction with the benedict\u2019s reagent<\/li>\r\n \t<li style=\"text-align: justify\">Hydrolysis of sucrose in an acidic solution or by enzyme invertase or sucraseresults in its monomer i.e. a molecule of fructoseandglucose<\/li>\r\n \t<li>Sucrose has ana-1,2 glycosidic linkage<\/li>\r\n<\/ul>\r\n&nbsp;\r\n\r\n<img class=\"aligncenter size-full wp-image-32\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-11.png\" alt=\"\" width=\"298\" height=\"153\" \/>\r\n\r\n<strong><em>Lactose<\/em><\/strong>\r\n<ul>\r\n \t<li style=\"text-align: justify\">Lactose is formed by the mammary glands and occurs to the extent of about 5 per cent in milk hence it is called as milk sugar<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li>Lactose is hydrolyzed by acids and the specific enzyme lactase into its constituent monosaccharides glucose and galactose<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li>It is reducing sugar forms osazones, a cyanhydrin and an oxime and is decomposed by alkali<\/li>\r\n<\/ul>\r\n<ul>\r\n \t<li style=\"text-align: justify\">Lactose intolerance is a fairly common condition means deficiency of the lactase enzymewhich breaks glycosidic bond<\/li>\r\n<\/ul>\r\n<ul>\r\n \t<li style=\"text-align: justify\">Lactose has an b-1,4 glycosidic linkage<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li>A water molecule and lactose is formed with the dehydration of monomeric unit one a-d-glucose and one b-d-galactose<\/li>\r\n<\/ul>\r\n<ul>\r\n \t<li style=\"text-align: justify\">Human milk differs fromcow\u2019s milk in containing in addition to regular lactose, other oligosaccharides such as L-fucosyl-lactose, in which the deoxy sugar L-fucose is linked to galactose at carbon 2<\/li>\r\n<\/ul>\r\n<img class=\"aligncenter size-full wp-image-33\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-12.png\" alt=\"\" width=\"269\" height=\"155\" \/>\r\n\r\n<strong><em>Maltose<\/em><\/strong>\r\n<ul>\r\n \t<li>Malt sugar<\/li>\r\n<\/ul>\r\n<ul>\r\n \t<li style=\"text-align: justify\">Maltose is made of two glucose units and is formed when the enzyme amylase or diastase hydrolyzes starch.<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li>Maltose is formed as an intermediate product in the acid hydrolysis of starch and consequently is an important constituent of corn syrups, which are prepared by partial hydrolysis of starch with dilute acid<\/li>\r\n<\/ul>\r\n<ul>\r\n \t<li style=\"text-align: justify\">Maltose is readily fermented by yeast<\/li>\r\n<\/ul>\r\n<ul>\r\n \t<li>Maltose has an a-1,4 linkage<\/li>\r\n<\/ul>\r\n&nbsp;\r\n\r\n<img class=\"aligncenter size-full wp-image-34\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-13.png\" alt=\"\" width=\"262\" height=\"150\" \/>\r\n\r\n&nbsp;\r\n\r\n<strong><em>Cellobiose<\/em><\/strong>\r\n<ul>\r\n \t<li style=\"text-align: justify\">Cellobiose is obtained as a product of the incomplete hydrolysis of cellulose<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li>It is composed to two molecules of glucose into which it is split by either acids or the enzyme emulsion<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li>It is reducing sugar and give the reaction characteristic of the sugar group<\/li>\r\n<\/ul>\r\n<ul style=\"text-align: justify\">\r\n \t<li>Cellobiose bears the same relation to cellulose as maltose does to starch and glycogen<\/li>\r\n<\/ul>\r\n<p style=\"text-align: justify\"><img class=\"aligncenter size-full wp-image-35\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-14.png\" alt=\"\" width=\"354\" height=\"190\" \/><\/p>\r\n&nbsp;\r\n\r\n&nbsp;\r\n<table>\r\n<tbody>\r\n<tr>\r\n<td><strong>you can view video on Prelude: Carbohydrates (Part-A) \u2013Mono &amp; Oligosaccharides<\/strong><\/td>\r\n<td><a href=\"https:\/\/youtu.be\/mW1nQkhQSIA\" target=\"_blank\" rel=\"noopener\"><img class=\"alignnone wp-image-120\" src=\"http:\/\/epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/2018\/11\/download.png\" alt=\"\" width=\"36\" height=\"36\" \/><\/a><\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<div><\/div>\r\n<div><\/div>\r\n<div class=\"textbox learning-objectives\">\r\n\r\n\r\n\r\n\r\n\r\n<h3><strong>References<\/strong><\/h3>\r\n<ul>\r\n \t<li style=\"text-align: justify\">Manoli, F.. \"Spontaneous precipitation of calcium carbonate in the presence of chondroitin sulfate\", Journal of Crystal Growth, 2000080<\/li>\r\n<\/ul>\r\n&nbsp;\r\n\r\n<strong>\u00a0 \u00a0 Web site<\/strong>\r\n<ul>\r\n \t<li><a href=\"http:\/\/www.innovateus.net\/\">www.innovateus.net<\/a><\/li>\r\n \t<li>www. digitallibrary.srmuniv.ac.in<\/li>\r\n \t<li><a href=\"http:\/\/www.freepatentsonline.com\/\">www.freepatentsonline.com<\/a><\/li>\r\n \t<li><a href=\"http:\/\/www.cgcc.cc.or.us\/\">www.cgcc.cc.or.us<\/a><\/li>\r\n \t<li><a href=\"http:\/\/www.cheque.uq.edu.au\/\">www.cheque.uq.edu.au<\/a><\/li>\r\n \t<li>www.lrd.yahooapis.com<\/li>\r\n<\/ul>\r\n&nbsp;\r\n\r\n<strong>\u00a0 \u00a0Books<\/strong>\r\n<ul>\r\n \t<li style=\"text-align: justify\">Lehninger Principles of Biochemistry by David L. Nelson and Michael M. Cox 4th Edition<\/li>\r\n \t<li style=\"text-align: justify\">Instant Notes Biochemistry by B. D. Hames &amp; N. M. Hooper, 2nd Edition<\/li>\r\n \t<li style=\"text-align: justify\">Biochemistry, Jeremy M. Berg, John L. Tymoczko and Lubert Stryer, 5th Edition<\/li>\r\n<\/ul>\r\n<\/div>\r\n&nbsp;","rendered":"<div><span style=\"float: right\"><a href=\"https:\/\/youtu.be\/mW1nQkhQSIA\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" src=\"http:\/\/epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/2018\/11\/download.png\" alt=\"epgp books\" width=\"75px\" height=\"75px;\" \/><\/a><br \/>\n<\/span><\/div>\n<p><strong>\u00a0 \u00a0<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong> PRELUDE-CARBOHYDRATES-<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>(Monosaccharide\u2019s and Oligosaccharides)<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>Objectives<\/strong><\/p>\n<ol>\n<li>Introduction and the function of Functions of carbohydrates<\/li>\n<li>To understand classification of carbohydrates<\/li>\n<li>To study the characteristics of carbohydrates<\/li>\n<li>To understand the properties of mono- and di- saccharides:<\/li>\n<\/ol>\n<p><strong>\u00a0 \u00a0<\/strong><\/p>\n<p><strong>Introduction<\/strong><\/p>\n<ul>\n<li style=\"text-align: justify\">Carbohydrates are one of the three major macronutrients in our diet representing a wide group of substances which include the sugars, starches, glycogen, gums and celluloses. Carbohydrates are known as the important energy source for living things.<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li style=\"text-align: justify\">They comes from the process of photosynthesis, an endothermic reaction in which condensation of carbon dioxide takes place and which requires light energy and the pigment chlorophyll.<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li style=\"text-align: justify\">The chemical component of carbohydrates is carbon, hydrogen, and oxygen.<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li style=\"text-align: justify\">Generally, but not always the hydrogen and oxygen in carbohydrates are present in the proportion oftwo hydrogen atoms to one oxygen atom as in H2O, from which fact the term \u201ccarbohydrates\u201d was derived<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li style=\"text-align: justify\">The carbohydrates are actually or potentially hydroxy aldehydes or ketones, and in most cases they are polyhydroxy aldehyde or ketones compounds that can be hydrolyzed to them. It also means \u201chydrate of carbon\u201d<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li style=\"text-align: justify\">Plant biomass is made up of three fourths of carbohydrates but in animal body it is present in small quantities. In animal it is stored in the form of glycogen, sugar and their derivatives<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li>All living organism contains derived monosaccharides such as the sugar acids, amino sugars and the deoxysugars<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li>Derivatives of the carbohydrates also contain nitrogen, phosphates and sulfur compounds. Carbohydrates can combine with lipid and protein to form glycolipids and glycoproteins.<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li style=\"text-align: justify\">Carbohydrates and their derivatives are present in all animal tissues and tissue fluids, blood and milk. The glucosides are a class of carbohydrates are derivatives which are frequent constituents of plants<\/li>\n<\/ul>\n<ul>\n<li style=\"text-align: justify\">The major components of living matter is formed from carbohydrates, proteins and fats<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li style=\"text-align: justify\">This complex form of carbohydrates is required for the maintenance of cell structure and functions<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li>Presence of functional group like alcohol on almost every carbon makes the chemistry of carbohydrates a complex structure<\/li>\n<\/ul>\n<ul>\n<li style=\"text-align: justify\">Carbohydrate may be exist in either a straight chain or a closed ring structure. The general reaction between the aldehyde and ketone part of the carbohydrates and alcohol groups forms the derivative called hemiacetals and hemiketals leads to the formation of ring structure. The most common basic structure of carbohydrates is closed rings Glucose is an example of a single closed ring carbohydrate<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>Functions of carbohydrates<\/strong><\/p>\n<ul>\n<li style=\"text-align: justify\">The simplest carbohydrates are initially synthesized in plants by the process of photosynthesis which is a series of reactions.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>Carbohydrate functions as\u2026<\/strong><\/p>\n<ul>\n<li>bio Fuel<\/li>\n<li>primary Source of Energy<\/li>\n<li>storage food<\/li>\n<li>framework in body<\/li>\n<li>anticoagulant<\/li>\n<li>antigen<\/li>\n<li>hormone<\/li>\n<li>provide raw material for industry<\/li>\n<li>Essential for Fat Oxidation<\/li>\n<li>plays role in gastro-intestinal function<\/li>\n<li>add flavour to the diet<\/li>\n<li>play an important role in molecular recognition<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Classification<\/strong><\/p>\n<ul>\n<li style=\"text-align: justify\">The carbohydrates are sometimes referred to as the saccharides<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li>The word saccharides comes from the Greek word sakcharon meaning sugar<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li style=\"text-align: justify\">Those carbohydrates such as glucose and fructose that cannot be hydrolyzed into simpler compounds are called simple sugars or <em>monosaccharides<\/em><\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li style=\"text-align: justify\">Certain carbohydrates are made of two molecules of monosaccharides such as sucrose, lactose and maltose<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li>These carbohydrates are called <em>dissarcharides.<\/em> Similarly, trisaccharides are composed of three and tetrasaccharides of four monosaccharides molecules<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li style=\"text-align: justify\">All these compound carbohydrates may be readily hydrolyzed into their constituent monosaccharides by heating with dilute acids or by the action of specific enzymes<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li style=\"text-align: justify\">According to the number of C atoms and functional groups in its simplest form of carbohydrates are further classified<\/li>\n<\/ul>\n<ul>\n<li style=\"text-align: justify\">The suffix \u2013ose indicates the types of sugar.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong><em>Nomenclature of carbohydrates based on number of carbon atoms<\/em><\/strong><\/p>\n<ol>\n<li>Triose= 3 carbon atom(Glyceraldehyde, Dihydroxyacetone<\/li>\n<li>Tetrose= 4 carbon atom(Erythrose)<\/li>\n<li>Pentose= 5 carbon atom(Ribose, Ribulose, Xylulose)<\/li>\n<li>Hexose=6 carbon atom(Glucose, Galactose, Mannose, Fructose)<\/li>\n<li>Heptose=7 carbon atom(Sedoheptulose) and so on<\/li>\n<\/ol>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-21\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1.png\" alt=\"\" width=\"559\" height=\"309\" srcset=\"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1.png 559w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-300x166.png 300w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-65x36.png 65w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-225x124.png 225w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-350x193.png 350w\" sizes=\"auto, (max-width: 559px) 100vw, 559px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p><strong>Characteristics of carbohydrates<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong><em>Isomerism<\/em><\/strong><\/p>\n<p>&nbsp;<\/p>\n<p style=\"text-align: justify\">Isomers are defined as molecule with the same chemical formula and often with the same kinds of chemical bonds between atoms, but in which the atoms are arranged differently i.e. they have different structural formulae. Isomerism can be of two types: Structural isomerism and Stereoisomerism<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-39\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-15.png\" alt=\"\" width=\"500\" height=\"298\" srcset=\"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-15.png 500w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-15-300x179.png 300w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-15-65x39.png 65w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-15-225x134.png 225w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-15-350x209.png 350w\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p><strong>Monosaccharides <\/strong>Greek, <em>mono<\/em>=one; <em>sakchron<\/em>=sugar.<\/p>\n<ul>\n<li style=\"text-align: justify\">Monosaccharides are the simplest sugar formed by the single molecules which furthercannot be hydrolyzed into smaller units. Depending upon the number of carbon arrangement in a unit, mono saccharides are subdivided into aldoses and ketoses.<\/li>\n<\/ul>\n<ul>\n<li>The aldoses and ketoses are represented by the following general formulas.<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-23\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-2.png\" alt=\"\" width=\"349\" height=\"237\" srcset=\"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-2.png 349w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-2-300x204.png 300w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-2-65x44.png 65w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-2-225x153.png 225w\" sizes=\"auto, (max-width: 349px) 100vw, 349px\" \/><\/p>\n<ul>\n<li style=\"text-align: justify\">Study of the chemical and physical properties of many sugar has shown the cyclic forms predominate over open chain structure, both in solution and in the solid state.<\/li>\n<\/ul>\n<ul>\n<li style=\"text-align: justify\">Alcohol may react with aldehyde and ketone moieties of the carbohydrates to form an unstable compounds called hemiacetals or hemiketals, respectively. This is a reaction between two molecules, i.e., it is intermolecular<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-24\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-3.png\" alt=\"\" width=\"379\" height=\"214\" srcset=\"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-3.png 379w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-3-300x169.png 300w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-3-65x37.png 65w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-3-225x127.png 225w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-3-350x198.png 350w\" sizes=\"auto, (max-width: 379px) 100vw, 379px\" \/><\/p>\n<ul>\n<li style=\"text-align: justify\">In glucose the \u2013OH on carbon 5 (C-5) can react intramolecularly with carbonyl group (on C-1) to form a stable, cyclic hemiacetal. The cyclic form of glucose is a six-membered ring; such sugars are called <strong>pyranose<\/strong> because they resemble pyran. Fructose forms a five membered ring called <strong>furanose<\/strong> as it resembles furan.<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-25\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-4.png\" alt=\"\" width=\"324\" height=\"131\" srcset=\"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-4.png 324w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-4-300x121.png 300w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-4-65x26.png 65w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-4-225x91.png 225w\" sizes=\"auto, (max-width: 324px) 100vw, 324px\" \/><\/p>\n<ul>\n<li style=\"text-align: justify\">The pyranose and furanose ring structure of sugar proposed by Haworth more accurately represent their actual configuration than do the older projection structures used by Fischer and other workers, though the older ring forms are still used extensively in writing reaction of sugars.<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-26\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-5.png\" alt=\"\" width=\"368\" height=\"212\" srcset=\"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-5.png 368w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-5-300x173.png 300w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-5-65x37.png 65w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-5-225x130.png 225w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-5-350x202.png 350w\" sizes=\"auto, (max-width: 368px) 100vw, 368px\" \/><\/p>\n<ul>\n<li style=\"text-align: justify\">The pyranose ring of a six carbon aldose sugar can exist in either a boat or a chair configuration<\/li>\n<\/ul>\n<ul>\n<li style=\"text-align: justify\">The substituents attached to the ring carbon thatextend parallel to the symmetry are said to be axial (a) whereas those that extend outward from the axis are said to be equatorial (e).<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-27\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-6.png\" alt=\"\" width=\"420\" height=\"177\" srcset=\"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-6.png 420w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-6-300x126.png 300w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-6-65x27.png 65w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-6-225x95.png 225w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-6-350x148.png 350w\" sizes=\"auto, (max-width: 420px) 100vw, 420px\" \/><\/p>\n<p><strong><em>Stereoisomerism<\/em><\/strong><\/p>\n<ul>\n<li style=\"text-align: justify\">Many of the carbohydrates contain the same number of atoms and the same kinds of groups, yet definitely distinct substances<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li style=\"text-align: justify\">For example, the formula C6H12O6 represents 16 differently simple sugars all processing the structure CH2OH.CHOH.CHOH.CHOH.CHOH.CHO<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li style=\"text-align: justify\">This is due to differently arrangement of the constituent groups of the molecules in space. This phenomenon represents what is called stereoisomerism (space isomerism) and these sugars are stereoisomers.<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li>An asymmetric carbon atom is a carbon when it is attached to four different types of atoms or groups<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li style=\"text-align: justify\">When there are several asymmetric carbon atoms in a chain molecule and the end group<span style=\"text-align: initial;font-size: 1em\"> are not identical, the number of stereoisomers possible is equal to 2n, where n is the number of asymmetric carbon atoms<\/span><\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li style=\"text-align: justify\">For example glucose contains 4 asymmetric carbon and thus are 24= 16 isomers. The simplest monosaccharides is glyceraldehydes<\/li>\n<\/ul>\n<ul>\n<li style=\"text-align: justify\">Glyceraldehyde has a single asymmetric carbon atom (the central one) and so two <strong>stereoisomers <\/strong>(also called<strong> optical isomers<\/strong>) are possible, that is two forms of glyceraldehyde, denoted as <strong>D<\/strong>&#8211; and <strong>L-glyceraldehyde<\/strong>, which are mirror images of each other<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-28\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-7.png\" alt=\"\" width=\"320\" height=\"191\" srcset=\"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-7.png 320w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-7-300x179.png 300w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-7-65x39.png 65w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-7-225x134.png 225w\" sizes=\"auto, (max-width: 320px) 100vw, 320px\" \/><\/p>\n<p>&nbsp;<\/p>\n<ul>\n<li style=\"text-align: justify\">It has been possible to relate the spatial configuration of sugars, amino acids and many other optically active substances of biological importance to the configuration of the dextro and levo forms of glyceraldehydes<\/li>\n<\/ul>\n<ul>\n<li style=\"text-align: justify\">Monosaccharides can be named by a system that is based on the number of carbons with the suffix \u2013ose added. There is also a system for numbering the carbons. They are numbered sequentially, with the aldehyde or ketone group being on the carbon with the lowest possible number<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong><em>Optical isomerism<\/em><\/strong><\/p>\n<ul>\n<li style=\"text-align: justify\">When a beam of plane polarized lightthrough the solution of a non-racemic mixture, rotation of polarization occurs<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li>Carbohydrates contain asymmetric carbons, because of this carbohydrates are optically active, and rotating the plane polarized light<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li style=\"text-align: justify\">The compounds which rotates the plane polarized light to the right (clockwise) is called dextrorotatory (indicated by d or +)and the compound which rotates the plane polarized light to the left (counter clockwise) is called levorotatory (indicated by l or -).<\/li>\n<\/ul>\n<p style=\"text-align: justify\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-29\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-8.png\" alt=\"\" width=\"587\" height=\"230\" srcset=\"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-8.png 587w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-8-300x118.png 300w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-8-65x25.png 65w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-8-225x88.png 225w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-8-350x137.png 350w\" sizes=\"auto, (max-width: 587px) 100vw, 587px\" \/><\/p>\n<p><strong><em>Enantiomers<\/em><\/strong><\/p>\n<ul>\n<li style=\"text-align: justify\">An Enantiomers is one of two stereoisomers that are mirror images of each other and are non- superimposable<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-30\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-9.png\" alt=\"\" width=\"526\" height=\"210\" srcset=\"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-9.png 526w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-9-300x120.png 300w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-9-65x26.png 65w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-9-225x90.png 225w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-9-350x140.png 350w\" sizes=\"auto, (max-width: 526px) 100vw, 526px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p><strong><em>Epimers<\/em><\/strong><\/p>\n<ul>\n<li style=\"text-align: justify\">Epimers are those sugars which differ with its isomers in configuration at only one asymmetric carbon<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li>D-glucose and D-galactose contains four asymmetric groups, and that all these groups are identical except the configuration at C-4<\/li>\n<\/ul>\n<ul>\n<li style=\"text-align: justify\">Such isomers are called are epimersTheprocess of interconversion of epimers is known as epimerization and <em>epimerases<\/em> catalyzes this reaction<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-31\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-10.png\" alt=\"\" width=\"224\" height=\"238\" srcset=\"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-10.png 224w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-10-65x69.png 65w\" sizes=\"auto, (max-width: 224px) 100vw, 224px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p><strong>Properties of Mono saccharides:<\/strong><\/p>\n<ul>\n<li>Mutarotation<\/li>\n<li>Glycoside Formation<\/li>\n<li>Reducing Power<\/li>\n<li>Reduction<\/li>\n<li>Oxidation with mild and strong oxidizing agent<\/li>\n<li>Methylation \/ Esterification<\/li>\n<li>Dehydration<\/li>\n<li>Osazoneformation with phenyl hydrazine.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong><em>Glucose<\/em><\/strong><\/p>\n<ul>\n<li>Glucose is an aldohexose \u2013<\/li>\n<li>It is a poly hydroxyaldehyde and contains 6-carbon<\/li>\n<li style=\"text-align: justify\">Carbohydrates are transformedinto simplest glucose which is the primary carbohydrates utilized by the body tissues.<\/li>\n<li>Glucose is the sugar of blood and other body fluids<\/li>\n<li style=\"text-align: justify\">Disaccharide like sucrose, maltose and lactose and polysaccharides such as starch, cellulose and glycogen contains glucose as one of the monomers<\/li>\n<li>Glucose is 25% less sweet than sucrose<\/li>\n<li>Blood normally contains from 60 to 90 mg of glucose in each 100ml<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong><em>Galactose<\/em><\/strong><\/p>\n<ul>\n<li>Galactose is an aldohexose<\/li>\n<li>It is a poly hydroxyaldehyde and a 6-carbon containing compound<\/li>\n<li>It is found in dairy products, sugar beets, pectin and gum<\/li>\n<li>Galactose is one of the monomer of the disaccharide lactose<\/li>\n<li>Galactose is 80% less sweet than sucrose<\/li>\n<li>It is epimers of glucose<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong><em>Fructose<\/em><\/strong><\/p>\n<ul>\n<li>Fructose is a ketohexose<\/li>\n<li>It is a poly hydroxyketone and contains 6-carbon<\/li>\n<li>It is found in fruit, flowers, root vegetables and honey<\/li>\n<li>Disaccharide sucrose contains fructose as one monomer<\/li>\n<li>It is the sweetest of all naturally occurring carbohydrates and 175% sweeter than sucrose<\/li>\n<li>Fructose has higher solubility than other sugars<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>Properties of oligosaccharides<\/strong><\/p>\n<ul>\n<li style=\"text-align: justify\">The oligosaccharides are composed of the disaccharides, trisaccharides and tetrasaccharides and are so designated to indicate the number of monosaccharides units involved in the structures<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li style=\"text-align: justify\">Disaccharides is a sugar formed when a bond between the first carbon of one sugar and a hydroxyl group at any position on the other sugar is formed and a molecule of water is removed<\/li>\n<\/ul>\n<ul>\n<li style=\"text-align: justify\">Sucrose, maltose, and lactose are the most important disaccharides. Each one of these on hydrolysis with either an acid or an enzyme gives two molecules of the monosaccharides by which it is made of.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong><em>Sucrose<\/em><\/strong><\/p>\n<ul>\n<li style=\"text-align: justify\">It is called as table sugar<\/li>\n<li style=\"text-align: justify\">Sucrose occurs especially in the juices of plants such as sugar beets, sugar cane sorgum, sugar maple and pine apple<\/li>\n<li style=\"text-align: justify\">Ripe fruits are rich in sucrose<\/li>\n<li style=\"text-align: justify\">20% of sugar cane is sucrose<\/li>\n<li style=\"text-align: justify\">It is non-reducing sugar, is relatively stable toward the action of alkali and in general does not give the reaction characteristic of the sugar groupand hence no reaction with the benedict\u2019s reagent<\/li>\n<li style=\"text-align: justify\">Hydrolysis of sucrose in an acidic solution or by enzyme invertase or sucraseresults in its monomer i.e. a molecule of fructoseandglucose<\/li>\n<li>Sucrose has ana-1,2 glycosidic linkage<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-32\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-11.png\" alt=\"\" width=\"298\" height=\"153\" srcset=\"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-11.png 298w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-11-65x33.png 65w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-11-225x116.png 225w\" sizes=\"auto, (max-width: 298px) 100vw, 298px\" \/><\/p>\n<p><strong><em>Lactose<\/em><\/strong><\/p>\n<ul>\n<li style=\"text-align: justify\">Lactose is formed by the mammary glands and occurs to the extent of about 5 per cent in milk hence it is called as milk sugar<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li>Lactose is hydrolyzed by acids and the specific enzyme lactase into its constituent monosaccharides glucose and galactose<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li>It is reducing sugar forms osazones, a cyanhydrin and an oxime and is decomposed by alkali<\/li>\n<\/ul>\n<ul>\n<li style=\"text-align: justify\">Lactose intolerance is a fairly common condition means deficiency of the lactase enzymewhich breaks glycosidic bond<\/li>\n<\/ul>\n<ul>\n<li style=\"text-align: justify\">Lactose has an b-1,4 glycosidic linkage<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li>A water molecule and lactose is formed with the dehydration of monomeric unit one a-d-glucose and one b-d-galactose<\/li>\n<\/ul>\n<ul>\n<li style=\"text-align: justify\">Human milk differs fromcow\u2019s milk in containing in addition to regular lactose, other oligosaccharides such as L-fucosyl-lactose, in which the deoxy sugar L-fucose is linked to galactose at carbon 2<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-33\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-12.png\" alt=\"\" width=\"269\" height=\"155\" srcset=\"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-12.png 269w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-12-65x37.png 65w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-12-225x130.png 225w\" sizes=\"auto, (max-width: 269px) 100vw, 269px\" \/><\/p>\n<p><strong><em>Maltose<\/em><\/strong><\/p>\n<ul>\n<li>Malt sugar<\/li>\n<\/ul>\n<ul>\n<li style=\"text-align: justify\">Maltose is made of two glucose units and is formed when the enzyme amylase or diastase hydrolyzes starch.<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li>Maltose is formed as an intermediate product in the acid hydrolysis of starch and consequently is an important constituent of corn syrups, which are prepared by partial hydrolysis of starch with dilute acid<\/li>\n<\/ul>\n<ul>\n<li style=\"text-align: justify\">Maltose is readily fermented by yeast<\/li>\n<\/ul>\n<ul>\n<li>Maltose has an a-1,4 linkage<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-34\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-13.png\" alt=\"\" width=\"262\" height=\"150\" srcset=\"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-13.png 262w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-13-65x37.png 65w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-13-225x129.png 225w\" sizes=\"auto, (max-width: 262px) 100vw, 262px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p><strong><em>Cellobiose<\/em><\/strong><\/p>\n<ul>\n<li style=\"text-align: justify\">Cellobiose is obtained as a product of the incomplete hydrolysis of cellulose<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li>It is composed to two molecules of glucose into which it is split by either acids or the enzyme emulsion<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li>It is reducing sugar and give the reaction characteristic of the sugar group<\/li>\n<\/ul>\n<ul style=\"text-align: justify\">\n<li>Cellobiose bears the same relation to cellulose as maltose does to starch and glycogen<\/li>\n<\/ul>\n<p style=\"text-align: justify\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-35\" src=\"http:\/\/biocp04.epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/sites\/215\/2019\/04\/1-14.png\" alt=\"\" width=\"354\" height=\"190\" srcset=\"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-14.png 354w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-14-300x161.png 300w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-14-65x35.png 65w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-14-225x121.png 225w, https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-content\/uploads\/sites\/215\/2019\/04\/1-14-350x188.png 350w\" sizes=\"auto, (max-width: 354px) 100vw, 354px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<table>\n<tbody>\n<tr>\n<td><strong>you can view video on Prelude: Carbohydrates (Part-A) \u2013Mono &amp; Oligosaccharides<\/strong><\/td>\n<td><a href=\"https:\/\/youtu.be\/mW1nQkhQSIA\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-120\" src=\"http:\/\/epgpbooks.inflibnet.ac.in\/wp-content\/uploads\/2018\/11\/download.png\" alt=\"\" width=\"36\" height=\"36\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<div><\/div>\n<div><\/div>\n<div class=\"textbox learning-objectives\">\n<h3><strong>References<\/strong><\/h3>\n<ul>\n<li style=\"text-align: justify\">Manoli, F.. &#8220;Spontaneous precipitation of calcium carbonate in the presence of chondroitin sulfate&#8221;, Journal of Crystal Growth, 2000080<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0 \u00a0 Web site<\/strong><\/p>\n<ul>\n<li><a href=\"http:\/\/www.innovateus.net\/\">www.innovateus.net<\/a><\/li>\n<li>www. digitallibrary.srmuniv.ac.in<\/li>\n<li><a href=\"http:\/\/www.freepatentsonline.com\/\">www.freepatentsonline.com<\/a><\/li>\n<li><a href=\"http:\/\/www.cgcc.cc.or.us\/\">www.cgcc.cc.or.us<\/a><\/li>\n<li><a href=\"http:\/\/www.cheque.uq.edu.au\/\">www.cheque.uq.edu.au<\/a><\/li>\n<li>www.lrd.yahooapis.com<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0 \u00a0Books<\/strong><\/p>\n<ul>\n<li style=\"text-align: justify\">Lehninger Principles of Biochemistry by David L. Nelson and Michael M. Cox 4th Edition<\/li>\n<li style=\"text-align: justify\">Instant Notes Biochemistry by B. D. Hames &amp; N. M. Hooper, 2nd Edition<\/li>\n<li style=\"text-align: justify\">Biochemistry, Jeremy M. Berg, John L. Tymoczko and Lubert Stryer, 5th Edition<\/li>\n<\/ul>\n<\/div>\n<p>&nbsp;<\/p>\n","protected":false},"author":3,"menu_order":1,"template":"","meta":{"pb_show_title":"on","pb_short_title":"","pb_subtitle":"","pb_authors":["dr-ramesh-kothari"],"pb_section_license":""},"chapter-type":[],"contributor":[58],"license":[],"class_list":["post-20","chapter","type-chapter","status-publish","hentry","contributor-dr-ramesh-kothari"],"part":3,"_links":{"self":[{"href":"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-json\/pressbooks\/v2\/chapters\/20","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-json\/pressbooks\/v2\/chapters"}],"about":[{"href":"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-json\/wp\/v2\/types\/chapter"}],"author":[{"embeddable":true,"href":"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-json\/wp\/v2\/users\/3"}],"version-history":[{"count":8,"href":"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-json\/pressbooks\/v2\/chapters\/20\/revisions"}],"predecessor-version":[{"id":48,"href":"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-json\/pressbooks\/v2\/chapters\/20\/revisions\/48"}],"part":[{"href":"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-json\/pressbooks\/v2\/parts\/3"}],"metadata":[{"href":"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-json\/pressbooks\/v2\/chapters\/20\/metadata\/"}],"wp:attachment":[{"href":"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-json\/wp\/v2\/media?parent=20"}],"wp:term":[{"taxonomy":"chapter-type","embeddable":true,"href":"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-json\/pressbooks\/v2\/chapter-type?post=20"},{"taxonomy":"contributor","embeddable":true,"href":"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-json\/wp\/v2\/contributor?post=20"},{"taxonomy":"license","embeddable":true,"href":"https:\/\/ebooks.inflibnet.ac.in\/biocp04\/wp-json\/wp\/v2\/license?post=20"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}